Regulatory
PDF1.2 (sh
Part:BBa_K2404002
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-09-22)
PDF1.2 shortened - a jasmonic acid-induced promoter
The PDF1.2 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to jasmonic acid.
It is a truncated version of BBa_K1467103
This promotor includes 500bp upstream of the PDF1.2 translational start point.
This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
This part is has been cloned into the P10500 plasmid using BsmBI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 408
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 517
[edit]
Categories
Parameters
None |